Who conducted an experiment using boiled broths in s-shaped containers to disprove spontaneous generation?

The experiment involving boiled broths in s-shaped containers is attributed to Louis Pasteur. He designed this experiment to investigate the theory of spontaneous generation, which suggested that living organisms could arise from non-living matter. By using s-shaped flasks, Pasteur was able to allow air to enter while preventing dust and microorganisms from contaminating the broth. After boiling the broth to kill any preexisting microbes, he observed that no microbial growth occurred in the flasks, demonstrating that microorganisms in the air were responsible for contaminating the broth. This provided strong evidence against the notion of spontaneous generation and supported the idea of biogenesis, which states that life arises from existing life. Pasteur's work had a profound impact on microbiology and our understanding of infectious diseases.