Which of the following techniques is primarily used to generate a genetic profile?

DNA fingerprinting is the technique primarily used to generate a genetic profile. This method analyzes specific regions of the DNA that are highly variable among individuals, known as short tandem repeats (STRs). By examining these unique patterns of DNA sequences, scientists can create a distinct genetic profile for each individual.

This technique is widely utilized in various fields, including forensics for solving crimes, paternity testing, and genetic diversity studies. It is especially valuable because it can identify individuals even from small samples of biological material, such as hair or saliva.

In contrast, DNA sequencing involves determining the exact order of nucleotides in a DNA molecule, which does provide information about the genetic makeup but is typically not used for profiling specific individuals. Gene therapy focuses on correcting or replacing defective genes in therapeutic contexts and is not related to profiling. RNA analysis examines gene expression levels but does not generate a genetic profile in the same way DNA fingerprinting does.