What method involves inserting foreign DNA directly into a cell's nucleus using a micropipet?

Micro-injection is a method that enables scientists to directly insert foreign DNA into a cell's nucleus using a micropipet. This precise technique involves creating a fine glass pipet that can penetrate the cell membrane without causing significant damage to the cell. The micropipet is used to inject DNA directly into the nucleus, allowing for efficient uptake and expression of the foreign genetic material within the target cell.

This method is particularly useful in the context of creating genetically modified organisms, as it allows for the introduction of specific genes into the genome of an organism with great accuracy. It is often employed in the fields of developmental biology and transgenics, where researchers require precise manipulation of cellular processes at the genetic level.

In contrast, the other methods listed serve different purposes. Electroporation involves applying an electric field to increase the permeability of the cell membrane, facilitating the uptake of DNA; however, it does not involve direct injection into the nucleus. Gene transfer is a broader term that can include various methods of introducing genetic material into cells, but it does not specify the technique used. Transformation typically refers to the uptake of DNA by bacteria, often through natural competence or induced methods, rather than the direct manipulation seen in micro-injection.